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The dynamics of change in HLA-DR expression on peripheral blood monocytes from patients with chronic myeloid leukemia (CML) who have undergone therapy with alpha-interferon (alpha-IFN) and Ara-C and the response to treatment were investigated. A modified Leukocyte Typing System (LTS) was used to identify HLA-DR isoenzymes in the patients’ peripheral blood. HLA-DR1-positive, DR4-negative monocytes were decreased in numbers and the DR3,4 monocyte population disappeared within 6 months after the start of treatment. A total of 33% of patients who were HLA-DR3,4 negative prior to treatment had become DR3,4 positive by 6 months after the beginning of treatment, and a total of 14% of patients had become HLA-DR1 positive. In contrast, the DR2,3 monocyte population decreased in numbers prior to the start of treatment, and the DR1,2 monocyte population increased in number. The majority of patients maintained DR3,3 positive monocytes throughout the duration of treatment, and the numbers of DR4 positive monocytes did not change significantly during treatment. Changes in HLA-DR isoenzyme expression could be used as an immunologic marker to monitor response to treatment in CML.
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A photosensitive member used in electrophotographic copiers is often subjected to exposure under a DC voltage condition when used in such copiers and therefore has a potential of +1400 V or higher. This potential is a typical example of a so-called high-voltage potential.
In the process of making an electrophotographic photosensitive member, the photosensitive member is treated with electrical charging to a high-voltage potential (1400 V or higher). Such a potential is applied to the photosensitive member in a pre-treating process (e.g., the preliminary charging). This pre-treating process is conducted to reduce a residual potential remaining after the photosensitive member is exposed to light, and if applied to the photosensitive member under conditions different from the conditions for the charging treatment, would cause uneven charging, abnormal discharge and even abrasion of the photosensitive member due to the repeated application of electrical discharges. To obviate these problems, the pre-charging is effected under conditions to which the photosensitive member will be subjected in the