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In a previous study, we demonstrated that the mRNA of the progesterone receptor (PR) is expressed differently in microvillar (MV) and non-MV types of the human umbilical vein endothelial cells (HUVEC). Our aim was to analyze PR and estrogen receptor (ER) expression in these two types of the HUVEC. We examined the mRNA and protein expression of PR, ERα, and ERβ in two types of the HUVEC. The expression of PR mRNA in MV- and non-MV-type HUVEC was observed by reverse transcription-polymerase chain reaction and Western blot. The protein expression of PR in MV- and non-MV-type HUVEC was examined by immunofluorescence and immunocytochemistry. We found that HUVEC express PR and ERα. PR and ERα protein expression was higher in MV-type HUVEC than in non-MV-type HUVEC. Both MV- and non-MV-type HUVEC express ERβ. The expression of PR mRNA was observed in both types of the HUVEC. PR protein was expressed in the nucleus of both MV- and non-MV-type HUVEC. The expression of PR and ERα protein was higher in MV-type HUVEC than in non-MV-type HUVEC. The significance of our findings may be the explanation of the fact that the progesterone-based therapies are effective for the treatment of ovarian and endometrial cancer, although the efficacy of those therapies varies.2015 Luanda Grand Prix
The 2015 Luanda Grand Prix was a professional tennis tournament played on clay courts. It was the eighth edition of the tournament which was part of the 2015 ATP Challenger Tour. It took place in Luanda, Angola between 28 April and 4 May 2015.
Singles main draw entrants
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Operating System: Windows 7/8/8.1/10.
Display: All Display Type.
Memory: 512 MB or more to support video.
Hard Disk: 5 MB or more to support video and background sound.
Notebook: CPU: 1.6 GHz+ and AMD at least 1 Gb Memory.
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